GenWay/绵羊/山羊IgG抗体（FITC）/GWB-74034D/1 mg
商品编号：
GWB-74034D
品牌：
genwaybio
市场价：
¥11580.00
美元价：
8685.00
产品分类：
抗山羊
公司分类：
Goat_resistance
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:
Short Description: DONKEY ANTI SHEEP/GOAT IgGFITCAdditional Information:
| Name | Sheep/Goat IgG Antibody (FITC) |
|---|---|
| Related Product Names | DONKEY ANTI SHEEP/GOAT IgG: FITC IgGIGHG1 |
| Gene Symbol | IGHG3 |
| Gene Family | IGH |
| Gene id | 3502 |
| Alias Symbols | IgG3, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, MGC45809, DKFZp686H11213, IGHG3 |
| Description of Target | DONKEY ANTI SHEEP/GOAT IgG:FITC |
| Swissprot ID | P01860 |
| Species Reactivity | Sheep |
| Host | Donkey |
| Isotype | Polyclonal IgG |
| Conjugation | FITC |
| Replacement | This antibody may replace item sc-34663 from Santa Cruz Biotechnology. |
| Immunogen | Sheep IgG |
| Homology | Sheep |
| Product Format | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Specificity | IgG |
| Concentration | 1mg/ml |
| Applications | C |
| Application Info | Immunohistology - Frozen: 1/100 - 1/200 |
| Clonality | Polyclonal |
| Format | Solution: Phosphate buffered saline, Stabalizer: 0.09%, Sodium Azide. 1%, Bovine Serum Albumin, Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Stability | 18 months from date of despatch. |
| Reconstitution and Storage | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Reactivity | Sheep |
| Storage | Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this |
| Protocol Information | Citation: 1: Hallström T, Resman F, Ristovski M, Riesbeck K. Binding of complement regulators to invasive nontypeable Haemophilus influenzae isolates is not increased compared to nasopharyngeal isolates, but serum resistance is linked to disease severity. J Clin Microbiol. 2010 Mar;48(3):921-7. Epub 2010 Jan 20. PubMed PMID: 20089757; PubMed Central PMCID: PMC2832458. Species: Normal Human Serum(NHS) Experiment Name: Serum binding assay Experiment Background: In the present study, the characteristics of invasive nontypeable Haemophilus influenzae (NTHi) infections, including evidence of immune deficiency in the individual patient and the clinical presentation of the septic event, were studied. Teresia et al. correlated these findings with the capacity to bind specific complement regulators and the in vitro serum resistance of the individual isolates. Experimental Steps: 1. To analyze whether NTHi from different isolation sites bound C4BP or factor H directly from NHS, bacteria were grown overnight in BHI broth. NTHi bacteria (109) were incubated with hiNHS and buffer (100 mM NaCl, 50 mM Tris-HCl, pH 7.4) for 1 h at37°C. 2. To remove unbound proteins, bacteria were washed 5 times with the same buffer. Thereafter, the bacterial pellet was resuspended in 150 µl of 0.1 M glycine-HCl, pH 2.0, in order to elute bound proteins.3. Bacteria without NHS were used as a negative control. After 15 min of incubation at 37°C with shaking, bacteria were centrifuged and the supernatants were subjected to SDS-PAGE (10%).4. To analyze whether NTHi bound vitronectin from NHS, bacteria were grown overnight and incubated with hiNHS and PBS. To removeunbound proteins, NTHi bacteria were washed 5 times with the same buffer.5. Thereafter, the bacterial pellet was resuspended in 50 µl of 0.1% Triton X-100 (Darmstadt, Germany) and protease inhibitors (Complete; Roche, Mannheim, Germany).6. After 30 min of incubation at 4°C, bacteria were centrifuged and the supernatants were subjected to SDS-PAGE (10%).7. Electrophoretic transfer of protein bands from the gel to an Immobilon-P membrane (Millipore, Bedford, MA) was done at 35 V for 2 h. After transfer, the Immobilon-P membrane was blocked in PBS with 0.1% Tween 20 (PBS-Tween) containing 5% milk powder. After several washings, the membrane was incubated with rabbit anti-human C4BP, goat anti-human factor H, or goat anti-human vitronectin pAb, followed by incubation with HRP-conjugated swine anti-rabbit or donkey-anti-goat pAb.8. After incubation and additional washings in PBS-Tween, development was performed with enhanced chemiluminescence (ECL) Western blotting detection reagents (Pierce, Rockford, IL). Other Reagents Used: NaCl, glucose, gelatin, MgCl2 , CaCl2. Number Of Protocols: 1 |
| Lead Time | Domestic: within 2-3 weeks delivery International: 2-3 weeks |
| Intended Use | Research Use Only |
| Key Reference | 1. Singh, M. et al. (1999) A recombinant measles virus expressing hepatitis B virus surface antigen induces humoral immune responses in genetically modified mice. J. Virol. 73 (6): 4823 - 4828. 2. Tedla, N. et al. (1998) Regulation of T lymphocyte trafficking into lymph nodes during an immune response by the chemokines macrophage inflammatory protein (MIP) - 1 alpha and MIP-1 beta. J. Immunol. 161: 5663 - 5672 3. Turner, J. et al. (2002) in vivo IL-10 production reactivates chronic pulmonary tuberculosis in C57BL/6 mice. J. Immunol. 169: 6343 - 6351. |
| :: | Preservative Stabilisers: 0.09% - Sodium Azide 1% - Bovine Serum Albumin Antiserum Preparation: Antisera to sheep IgG were raised by repeated immunisation of donkeys with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
| :: | Approx Protein Conc: IgG concentration 1.0 mg/ml Buffer Solutions: Phosphate buffered saline pH7.2 |
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GenWay公司是为了探索和开发人类蛋白组而成立的。公司的总任务就是用基因特异性抗体來筛选和鉴定新蛋白、其功能及其编码基因，旨在成为以抗体介导的蛋白组研发的领域先锋。基因纬公司已成功地发展出一套有自己知识产权的、新穎、高效、低耗、高筛选通量的鉴定方法――抗体介导的蛋白和基因鉴定。公司提供大量的高质量的蛋白和相应的鸡抗体产品，是蛋白质组学研究的重要工具和有力助手。 作为领先的蛋白质和抗体解决方案供应商，GenWay 拥有专有技术平台和国际网络，可为我们的客户提供蛋白质、抗体、检测和细胞系的解决方案和应用。我们专注于重组蛋白和域特异性 IgY（鸡）抗体及其应用，例如 Seppro® 产品和难以表达的蛋白质。 GenWay 的技术和专业知识可制造高质量的蛋白质抗原，从而能够生产高质量的抗体和相关应用。GenWay Biotech的主要产品包括：ProteinsAntibodiesELISA KitsSecondary AntibodiesReagentsPeptidesMolecular Biology ProductsActive ProteinsEnzymatic AssaysPCR Kits
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