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当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > GenWay/马IgA ELISA/GWB-374Z31/96井
商品详细GenWay/马IgA ELISA/GWB-374Z31/96井
GenWay/马IgA ELISA/GWB-374Z31/96井
GenWay/马IgA ELISA/GWB-374Z31/96井
商品编号: GWB-374Z31
品牌: genwaybio
市场价: ¥9560.00
美元价: 7170.00
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Additional Information:

Name Equine IgA ELISA
Related Product Names IgA, Immunoglobulin A, Horse IgA, Equine Immunoglobulin A, Horse Immunoglobulin A ELISA
Alias Symbols IGHA1, IGHA2, IgA, Ig alpha 1 chain C region, Ig alpha 2 chain C region
Protein Name IgA
Species Reactivity Horse
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Sensitivity 3.654 ng/ml
Kit Range 15.63 ng/ml - 500 ng/ml
Homology Horse
Standard Range 500 ng/ml-15.63 ng/ml
Sample Type Serum, plasma and other biological fluids
Product Format In Solution
Specimen/Volumes Refer To Manual
Concentration Lot Specific
Applications ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-IgA conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-HORSE IgA ELISA MICRO PLATE
Anti-Horse IgA coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. HORSE IgA CALIBRATOR
Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity Equine
Kit Size 96 well
Kit Principle Genway Biotech IgA ELISA Kit (Horse) (GWB-374Z31) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for IgA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-IgA - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IgA captured in the well.
Kit Component
ComponentAmount
Anti-Horse IgA Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Horse IgA Calibrator1
Kit Duration 70 Minutes
Storage Refer to kit instructions for component storage
Lead Time Domestic: within 1 week delivery International: 1 week
Intended Use Research Use Only
Additional Information Intended Use: The total Horse IgA test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgA in horses’ biological samples.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Horse IgA antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HORSE IgA ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Horse IgA.
7. HORSE IgA CALIBRATOR
One vial containing Horse IgA calibrator.
品牌介绍
GenWay公司是为了探索和开发人类蛋白组而成立的。公司的总任务就是用基因特异性抗体來筛选和鉴定新蛋白、其功能及其编码基因,旨在成为以抗体介导的蛋白组研发的领域先锋。基因纬公司已成功地发展出一套有自己知识产权的、新穎、高效、低耗、高筛选通量的鉴定方法――抗体介导的蛋白和基因鉴定。公司提供大量的高质量的蛋白和相应的鸡抗体产品,是蛋白质组学研究的重要工具和有力助手。 作为领先的蛋白质和抗体解决方案供应商,GenWay 拥有专有技术平台和国际网络,可为我们的客户提供蛋白质、抗体、检测和细胞系的解决方案和应用。我们专注于重组蛋白和域特异性 IgY(鸡)抗体及其应用,例如 Seppro® 产品和难以表达的蛋白质。 GenWay 的技术和专业知识可制造高质量的蛋白质抗原,从而能够生产高质量的抗体和相关应用。GenWay Biotech的主要产品包括:ProteinsAntibodiesELISA KitsSecondary AntibodiesReagentsPeptidesMolecular Biology ProductsActive ProteinsEnzymatic AssaysPCR Kits